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Sample GSM1320754 Query DataSets for GSM1320754
Status Public on Feb 04, 2014
Title genomic DNA from Citrate, o/n at -80C Human whole blood (3021Cit80)
Sample type genomic
Source name Citrate, o/n at -80C Human whole blood 3021Cit80
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: Citrate, o/n at -80C
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description 3021Cit80
Citrate, o/n at -80C Human whole blood 3021Cit80
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9092802
foxp3DMR1in 0.9547287
foxp3DMRpro 0.9013875
cg00071250 0.4368609
cg00226923 0.952673
cg00431549 0.04041122
cg00491404 0.6787272
cg00546897 0.7512313
cg00554250 0.1593073
cg00620024 0.6583317
cg00673191 0.4672331
cg00739120 0.4365231
cg00795812 0.8435872
cg00899659 0.4904605
cg01031251 0.913114
cg01124420 0.4898123
cg01182697 0.3582212
cg01278291 0.5612152
cg01344171 0.7288825
cg01441777 0.5548161

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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