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Sample GSM1320753 Query DataSets for GSM1320753
Status Public on Feb 04, 2014
Title genomic DNA from B-cells Purified WBC subtype (B1877)
Sample type genomic
Source name B-cells Purified WBC subtype B1877
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: B-cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description B1877
B-cells Purified WBC subtype B1877
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9834586
foxp3DMR1in 0.9678531
foxp3DMRpro 0.9696418
cg00071250 0.743137
cg00226923 0.0952112
cg00431549 0.03899695
cg00491404 0.7806859
cg00546897 0.9127972
cg00554250 0.07060282
cg00620024 0.8559656
cg00673191 0.863871
cg00739120 0.1522708
cg00795812 0.7922313
cg00899659 0.2353272
cg01031251 0.9682941
cg01124420 0.5473428
cg01182697 0.1695561
cg01278291 0.06064146
cg01344171 0.2691782
cg01441777 0.63107

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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