GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1320750 Query DataSets for GSM1320750
Status Public on Feb 04, 2014
Title genomic DNA from Neutrophils Purified WBC subtype (Neut1147)
Sample type genomic
Source name Neutrophils Purified WBC subtype Neut1147
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Neutrophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Neut1147
Neutrophils Purified WBC subtype Neut1147
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.988207
foxp3DMR1in 0.9759794
foxp3DMRpro 0.9741049
cg00071250 0.9350139
cg00226923 0.9786353
cg00431549 0.0195801
cg00491404 0.8523668
cg00546897 0.479495
cg00554250 0.07198052
cg00620024 0.8872118
cg00673191 0.2175764
cg00739120 0.145045
cg00795812 0.9839482
cg00899659 0.0269441
cg01031251 0.8456027
cg01124420 0.7336004
cg01182697 0.0254884
cg01278291 0.200688
cg01344171 0.5810543
cg01441777 0.2698564

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap