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Sample GSM1320748 Query DataSets for GSM1320748
Status Public on Feb 04, 2014
Title genomic DNA from Eosinophils Purified WBC subtype (Eos2275)
Sample type genomic
Source name Eosinophils Purified WBC subtype Eos2275
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Eosinophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Eos2275
Eosinophils Purified WBC subtype Eos2275
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9896265
foxp3DMR1in 0.9812062
foxp3DMRpro 0.5302817
cg00071250 0.9442996
cg00226923 0.9810086
cg00431549 0.02241723
cg00491404 0.8593625
cg00546897 0.962074
cg00554250 0.1761222
cg00620024 0.9226006
cg00673191 0.02187317
cg00739120 0.1354837
cg00795812 0.9815319
cg00899659 0.02360698
cg01031251 0.8107525
cg01124420 0.7931585
cg01182697 0.052895
cg01278291 0.2201993
cg01344171 0.6686528
cg01441777 0.4489209

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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