NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1320747 Query DataSets for GSM1320747
Status Public on Feb 04, 2014
Title genomic DNA from Citrate, o/n at room temp Human whole blood (3021CitRt)
Sample type genomic
 
Source name Citrate, o/n at room temp Human whole blood 3021CitRt
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: Citrate, o/n at room temp
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
 
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description 3021CitRt
Citrate, o/n at room temp Human whole blood 3021CitRt
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
 
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
ID_REF
VALUE Average Beta (0-1 constrained), not normalized

Data table
ID_REF VALUE
cd3zDMR 0.913308
foxp3DMR1in 0.957091
foxp3DMRpro 0.8830456
cg00071250 0.4720172
cg00226923 0.9569698
cg00431549 0.06682413
cg00491404 0.7268592
cg00546897 0.7877221
cg00554250 0.1645938
cg00620024 0.5960193
cg00673191 0.5434127
cg00739120 0.4778199
cg00795812 0.8621046
cg00899659 0.5076736
cg01031251 0.9145537
cg01124420 0.4541205
cg01182697 0.3948638
cg01278291 0.6170556
cg01344171 0.7021174
cg01441777 0.6255675

Total number of rows: 96

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap