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Sample GSM1320745 Query DataSets for GSM1320745
Status Public on Feb 04, 2014
Title genomic DNA from Neutrophils Purified WBC subtype (Neut0612)
Sample type genomic
Source name Neutrophils Purified WBC subtype Neut0612
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Neutrophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Neut0612
Neutrophils Purified WBC subtype Neut0612
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9884924
foxp3DMR1in 0.9785013
foxp3DMRpro 0.9595132
cg00071250 0.95015
cg00226923 0.9789458
cg00431549 0.02698312
cg00491404 0.8665393
cg00546897 0.2386178
cg00554250 0.08703167
cg00620024 0.9521556
cg00673191 0.2205977
cg00739120 0.1525542
cg00795812 0.9855493
cg00899659 0.04821702
cg01031251 0.8537611
cg01124420 0.7333313
cg01182697 0.1593844
cg01278291 0.1964241
cg01344171 0.6711103
cg01441777 0.3620813

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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