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Sample GSM1320743 Query DataSets for GSM1320743
Status Public on Feb 04, 2014
Title genomic DNA from EDA, o/n at -80C Human whole blood (3045EDTA80)
Sample type genomic
Source name EDA, o/n at -80C Human whole blood 3045EDTA80
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: EDA, o/n at -80C
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description 3045EDTA80
EDA, o/n at -80C Human whole blood 3045EDTA80
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9615468
foxp3DMR1in 0.9719206
foxp3DMRpro 0.9570524
cg00071250 0.6233579
cg00226923 0.9678925
cg00431549 0.03990468
cg00491404 0.8453765
cg00546897 0.7568789
cg00554250 0.1406729
cg00620024 0.8272978
cg00673191 0.3313858
cg00739120 0.3124829
cg00795812 0.9409242
cg00899659 0.3076169
cg01031251 0.886662
cg01124420 0.6388706
cg01182697 0.2078509
cg01278291 0.4771461
cg01344171 0.6502528
cg01441777 0.5006623

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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