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Sample GSM1320740 Query DataSets for GSM1320740
Status Public on Feb 04, 2014
Title genomic DNA from Heparin, o/n at room temp Human whole blood (3177HepRt)
Sample type genomic
Source name Heparin, o/n at room temp Human whole blood 3177HepRt
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: Heparin, o/n at room temp
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description 3177HepRt
Heparin, o/n at room temp Human whole blood 3177HepRt
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.8773752
foxp3DMR1in 0.962714
foxp3DMRpro 0.8963524
cg00071250 0.3206682
cg00226923 0.9513273
cg00431549 0.05797863
cg00491404 0.7513373
cg00546897 0.8850816
cg00554250 0.158513
cg00620024 0.5544314
cg00673191 0.4788955
cg00739120 0.3477631
cg00795812 0.8209277
cg00899659 0.4497992
cg01031251 0.9135561
cg01124420 0.4295395
cg01182697 0.3033746
cg01278291 0.4688597
cg01344171 0.6912592
cg01441777 0.6954119

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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