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Sample GSM1320734 Query DataSets for GSM1320734
Status Public on Feb 04, 2014
Title genomic DNA from CD8+ T-cells Purified WBC subtype (Tc2636)
Sample type genomic
Source name CD8+ T-cells Purified WBC subtype Tc2636
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD8+ T-cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Tc2636
CD8+ T-cells Purified WBC subtype Tc2636
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.04489199
foxp3DMR1in 0.965106
foxp3DMRpro 0.7961611
cg00071250 0.03951082
cg00226923 0.9640118
cg00431549 0.059867
cg00491404 0.6504431
cg00546897 0.9737902
cg00554250 0.1578076
cg00620024 0.2650494
cg00673191 0.8462564
cg00739120 0.4924366
cg00795812 0.07420808
cg00899659 0.5116007
cg01031251 0.9591944
cg01124420 0.3130077
cg01182697 0.7255698
cg01278291 0.8866697
cg01344171 0.8398533
cg01441777 0.9483813

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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