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Sample GSM1320732 Query DataSets for GSM1320732
Status Public on Feb 04, 2014
Title genomic DNA from CD8- NK cells Purified WBC subtype (NK8m2641)
Sample type genomic
Source name CD8- NK cells Purified WBC subtype NK8m2641
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD8- NK cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NK8m2641
CD8- NK cells Purified WBC subtype NK8m2641
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.475556
foxp3DMR1in 0.9525191
foxp3DMRpro 0.9200382
cg00071250 0.06011336
cg00226923 0.9645337
cg00431549 0.02923747
cg00491404 0.2566512
cg00546897 0.9615791
cg00554250 0.1598856
cg00620024 0.9110284
cg00673191 0.6957213
cg00739120 0.2106007
cg00795812 0.8782661
cg00899659 0.4669161
cg01031251 0.9457469
cg01124420 0.5313794
cg01182697 0.2460749
cg01278291 0.7442352
cg01344171 0.7101814
cg01441777 0.664057

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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