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Sample GSM1320729 Query DataSets for GSM1320729
Status Public on Feb 04, 2014
Title genomic DNA from Tregs Purified WBC subtype (Treg1782)
Sample type genomic
Source name Tregs Purified WBC subtype Treg1782
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Tregs
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Treg1782
Tregs Purified WBC subtype Treg1782
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.05775112
foxp3DMR1in 0.4787208
foxp3DMRpro 0.2321364
cg00071250 0.03780349
cg00226923 0.9718794
cg00431549 0.1534931
cg00491404 0.6738099
cg00546897 0.9767413
cg00554250 0.3196155
cg00620024 0.1353276
cg00673191 0.8092518
cg00739120 0.6747216
cg00795812 0.08840272
cg00899659 0.9508797
cg01031251 0.9658915
cg01124420 0.2423737
cg01182697 0.7000859
cg01278291 0.9134707
cg01344171 0.7905372
cg01441777 0.928126

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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