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Sample GSM1320720 Query DataSets for GSM1320720
Status Public on Feb 04, 2014
Title genomic DNA from Basophils Purified WBC subtype (Baso1889)
Sample type genomic
Source name Basophils Purified WBC subtype Baso1889
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Basophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Baso1889
Basophils Purified WBC subtype Baso1889
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9798859
foxp3DMR1in 0.9658259
foxp3DMRpro 0.8295485
cg00071250 0.9054658
cg00226923 0.9378817
cg00431549 0.0440691
cg00491404 0.7955924
cg00546897 0.9617264
cg00554250 0.07958081
cg00620024 0.8264691
cg00673191 0.2536608
cg00739120 0.1535546
cg00795812 0.9760281
cg00899659 0.04093495
cg01031251 0.8821119
cg01124420 0.749378
cg01182697 0.08833747
cg01278291 0.6795561
cg01344171 0.4223683
cg01441777 0.3673308

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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