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Sample GSM1320719 Query DataSets for GSM1320719
Status Public on Feb 04, 2014
Title genomic DNA from CD8- NK cells Purified WBC subtype (NK8m4229)
Sample type genomic
Source name CD8- NK cells Purified WBC subtype NK8m4229
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD8- NK cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NK8m4229
CD8- NK cells Purified WBC subtype NK8m4229
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.1752893
foxp3DMR1in 0.9598226
foxp3DMRpro 0.8670531
cg00071250 0.03542459
cg00226923 0.9663574
cg00431549 0.03128843
cg00491404 0.2682523
cg00546897 0.9536561
cg00554250 0.1903225
cg00620024 0.8799004
cg00673191 0.8237251
cg00739120 0.3277313
cg00795812 0.9189625
cg00899659 0.6276574
cg01031251 0.9639793
cg01124420 0.4892716
cg01182697 0.3588779
cg01278291 0.787464
cg01344171 0.7148189
cg01441777 0.5843955

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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