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Sample GSM1320708 Query DataSets for GSM1320708
Status Public on Feb 04, 2014
Title genomic DNA from CD16- NK cells Purified WBC subtype (NK16m2578)
Sample type genomic
Source name CD16- NK cells Purified WBC subtype NK16m2578
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD16- NK cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NK16m2578
CD16- NK cells Purified WBC subtype NK16m2578
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.8954586
foxp3DMR1in 0.9726775
foxp3DMRpro 0.9363877
cg00071250 0.04146832
cg00226923 0.973321
cg00431549 0.02400924
cg00491404 0.1027104
cg00546897 0.9628071
cg00554250 0.1488955
cg00620024 0.8756357
cg00673191 0.8801727
cg00739120 0.1602675
cg00795812 0.9625694
cg00899659 0.3823605
cg01031251 0.9668507
cg01124420 0.7163262
cg01182697 0.1456401
cg01278291 0.8757411
cg01344171 0.7114682
cg01441777 0.4921066

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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