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Sample GSM1320707 Query DataSets for GSM1320707
Status Public on Feb 04, 2014
Title genomic DNA from CD16- NK cells Purified WBC subtype (NK16m2606)
Sample type genomic
Source name CD16- NK cells Purified WBC subtype NK16m2606
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD16- NK cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NK16m2606
CD16- NK cells Purified WBC subtype NK16m2606
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.8551422
foxp3DMR1in 0.9706993
foxp3DMRpro 0.9250579
cg00071250 0.05314247
cg00226923 0.9680439
cg00431549 0.02695595
cg00491404 0.08443987
cg00546897 0.969883
cg00554250 0.1244909
cg00620024 0.8197792
cg00673191 0.872144
cg00739120 0.1655802
cg00795812 0.9523729
cg00899659 0.4051176
cg01031251 0.9635183
cg01124420 0.6091346
cg01182697 0.1047128
cg01278291 0.7809287
cg01344171 0.7036113
cg01441777 0.4873432

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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