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Sample GSM1320704 Query DataSets for GSM1320704
Status Public on Feb 04, 2014
Title genomic DNA from NKT cells Purified WBC subtype (NKT2613)
Sample type genomic
Source name NKT cells Purified WBC subtype NKT2613
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: NKT cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NKT2613
NKT cells Purified WBC subtype NKT2613
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.2008999
foxp3DMR1in 0.8989871
foxp3DMRpro 0.7190109
cg00071250 0.05055287
cg00226923 0.9449002
cg00431549 0.05872796
cg00491404 0.3488327
cg00546897 0.9534208
cg00554250 0.2692794
cg00620024 0.5331601
cg00673191 0.7384853
cg00739120 0.7218432
cg00795812 0.1445918
cg00899659 0.8436826
cg01031251 0.927587
cg01124420 0.2624457
cg01182697 0.8635766
cg01278291 0.8831748
cg01344171 0.8702488
cg01441777 0.9237107

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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