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Sample GSM1320700 Query DataSets for GSM1320700
Status Public on Feb 04, 2014
Title genomic DNA from Basophils Purified WBC subtype (Baso1915)
Sample type genomic
Source name Basophils Purified WBC subtype Baso1915
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Basophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Baso1915
Basophils Purified WBC subtype Baso1915
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9871834
foxp3DMR1in 0.9738271
foxp3DMRpro 0.8881676
cg00071250 0.9090382
cg00226923 0.9760667
cg00431549 0.02263995
cg00491404 0.85184
cg00546897 0.9613037
cg00554250 0.07919785
cg00620024 0.8949001
cg00673191 0.319121
cg00739120 0.1273253
cg00795812 0.9792398
cg00899659 0.04068927
cg01031251 0.9218804
cg01124420 0.7132276
cg01182697 0.03647894
cg01278291 0.6615044
cg01344171 0.4513413
cg01441777 0.4271795

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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