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Sample GSM1320696 Query DataSets for GSM1320696
Status Public on Feb 04, 2014
Title genomic DNA from CD4+ T-cells Purified WBC subtype (Th2535)
Sample type genomic
Source name CD4+ T-cells Purified WBC subtype Th2535
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD4+ T-cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Th2535
CD4+ T-cells Purified WBC subtype Th2535
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.05151859
foxp3DMR1in 0.9253085
foxp3DMRpro 0.6841715
cg00071250 0.02982409
cg00226923 0.9626889
cg00431549 0.1633292
cg00491404 0.7173716
cg00546897 0.9675651
cg00554250 0.2469292
cg00620024 0.08694533
cg00673191 0.8341629
cg00739120 0.5593557
cg00795812 0.07081819
cg00899659 0.925392
cg01031251 0.9537194
cg01124420 0.06115357
cg01182697 0.7006637
cg01278291 0.8895425
cg01344171 0.7991956
cg01441777 0.9305617

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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