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Sample GSM1320693 Query DataSets for GSM1320693
Status Public on Feb 04, 2014
Title genomic DNA from Basophils Purified WBC subtype (Baso2477)
Sample type genomic
Source name Basophils Purified WBC subtype Baso2477
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Basophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Baso2477
Basophils Purified WBC subtype Baso2477
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9862633
foxp3DMR1in 0.9703808
foxp3DMRpro 0.9150317
cg00071250 0.9080881
cg00226923 0.9731643
cg00431549 0.02932012
cg00491404 0.8694383
cg00546897 0.9513335
cg00554250 0.08109026
cg00620024 0.8692661
cg00673191 0.4016715
cg00739120 0.1464004
cg00795812 0.9785227
cg00899659 0.0476379
cg01031251 0.9349392
cg01124420 0.6992429
cg01182697 0.04407698
cg01278291 0.5724933
cg01344171 0.5585818
cg01441777 0.369674

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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