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Sample GSM1320691 Query DataSets for GSM1320691
Status Public on Feb 04, 2014
Title genomic DNA from CD16- NK cells Purified WBC subtype (NK16m2640)
Sample type genomic
Source name CD16- NK cells Purified WBC subtype NK16m2640
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD16- NK cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NK16m2640
CD16- NK cells Purified WBC subtype NK16m2640
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.8964453
foxp3DMR1in 0.9725913
foxp3DMRpro 0.9490954
cg00071250 0.05361131
cg00226923 0.9727948
cg00431549 0.02428421
cg00491404 0.1283142
cg00546897 0.9645821
cg00554250 0.1350995
cg00620024 0.8778541
cg00673191 0.8260317
cg00739120 0.1351782
cg00795812 0.950082
cg00899659 0.4513741
cg01031251 0.9669712
cg01124420 0.5837203
cg01182697 0.1370793
cg01278291 0.8155944
cg01344171 0.7010541
cg01441777 0.4840493

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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