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Sample GSM1320686 Query DataSets for GSM1320686
Status Public on Feb 04, 2014
Title genomic DNA from EDA, o/n at -80C Human whole blood (3082EDTA80)
Sample type genomic
Source name EDA, o/n at -80C Human whole blood 3082EDTA80
Organism Homo sapiens
Characteristics sample type: Human whole blood
disease state: normal
sample subtype: EDA, o/n at -80C
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description 3082EDTA80
EDA, o/n at -80C Human whole blood 3082EDTA80
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9381549
foxp3DMR1in 0.9667773
foxp3DMRpro 0.9094952
cg00071250 0.5550817
cg00226923 0.9637206
cg00431549 0.05703061
cg00491404 0.8373923
cg00546897 0.7507294
cg00554250 0.1254231
cg00620024 0.6127104
cg00673191 0.3889862
cg00739120 0.3281525
cg00795812 0.9212483
cg00899659 0.3084017
cg01031251 0.881502
cg01124420 0.5025988
cg01182697 0.2151798
cg01278291 0.4560695
cg01344171 0.6874898
cg01441777 0.491513

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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