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Sample GSM1320682 Query DataSets for GSM1320682
Status Public on Feb 04, 2014
Title genomic DNA from Tregs Purified WBC subtype (Treg21691)
Sample type genomic
Source name Tregs Purified WBC subtype Treg21691
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Tregs
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Treg21691
Tregs Purified WBC subtype Treg21691
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.1304661
foxp3DMR1in 0.6597641
foxp3DMRpro 0.437166
cg00071250 0.04564949
cg00226923 0.9637835
cg00431549 0.1269259
cg00491404 0.6996567
cg00546897 0.9664659
cg00554250 0.2773384
cg00620024 0.1386709
cg00673191 0.8048927
cg00739120 0.6348542
cg00795812 0.1119654
cg00899659 0.8880622
cg01031251 0.9551654
cg01124420 0.186852
cg01182697 0.6670085
cg01278291 0.913523
cg01344171 0.8453326
cg01441777 0.8868949

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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