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Sample GSM1320679 Query DataSets for GSM1320679
Status Public on Feb 04, 2014
Title genomic DNA from NKT cells Purified WBC subtype (NKT2584)
Sample type genomic
Source name NKT cells Purified WBC subtype NKT2584
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: NKT cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NKT2584
NKT cells Purified WBC subtype NKT2584
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.4719236
foxp3DMR1in 0.9517708
foxp3DMRpro 0.7929357
cg00071250 0.09229683
cg00226923 0.9400305
cg00431549 0.09068847
cg00491404 0.7261052
cg00546897 0.9571422
cg00554250 0.2028861
cg00620024 0.1605008
cg00673191 0.8157477
cg00739120 0.3985655
cg00795812 0.1995433
cg00899659 0.7695939
cg01031251 0.954315
cg01124420 0.1693117
cg01182697 0.6389855
cg01278291 0.7835683
cg01344171 0.8213178
cg01441777 0.9064612

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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