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Sample GSM1320672 Query DataSets for GSM1320672
Status Public on Feb 04, 2014
Title genomic DNA from Monocytes Purified WBC subtype (Mono1775)
Sample type genomic
 
Source name Monocytes Purified WBC subtype Mono1775
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Monocytes
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
 
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Mono1775
Monocytes Purified WBC subtype Mono1775
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
 
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
ID_REF
VALUE Average Beta (0-1 constrained), not normalized

Data table
ID_REF VALUE
cd3zDMR 0.9844358
foxp3DMR1in 0.9775152
foxp3DMRpro 0.9339074
cg00071250 0.9035581
cg00226923 0.9539344
cg00431549 0.03174765
cg00491404 0.7590203
cg00546897 0.2341212
cg00554250 0.05376941
cg00620024 0.9174865
cg00673191 0.75793
cg00739120 0.1594641
cg00795812 0.5737471
cg00899659 0.03870953
cg01031251 0.9449933
cg01124420 0.7459945
cg01182697 0.05386052
cg01278291 0.4693586
cg01344171 0.3254525
cg01441777 0.3468764

Total number of rows: 96

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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