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Sample GSM1320667 Query DataSets for GSM1320667
Status Public on Feb 04, 2014
Title genomic DNA from Neutrophils Purified WBC subtype (Neut1145)
Sample type genomic
Source name Neutrophils Purified WBC subtype Neut1145
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Neutrophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Neut1145
Neutrophils Purified WBC subtype Neut1145
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9884325
foxp3DMR1in 0.9796507
foxp3DMRpro 0.9351718
cg00071250 0.935236
cg00226923 0.9732629
cg00431549 0.02076074
cg00491404 0.8897674
cg00546897 0.6266434
cg00554250 0.07051205
cg00620024 0.890449
cg00673191 0.2116075
cg00739120 0.1409994
cg00795812 0.983309
cg00899659 0.03270024
cg01031251 0.815982
cg01124420 0.7168148
cg01182697 0.03205454
cg01278291 0.1176924
cg01344171 0.5779652
cg01441777 0.3186623

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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