GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1320665 Query DataSets for GSM1320665
Status Public on Feb 04, 2014
Title genomic DNA from CD8- NK cells Purified WBC subtype (NK8m2299)
Sample type genomic
Source name CD8- NK cells Purified WBC subtype NK8m2299
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD8- NK cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NK8m2299
CD8- NK cells Purified WBC subtype NK8m2299
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.4322766
foxp3DMR1in 0.9667649
foxp3DMRpro 0.9402953
cg00071250 0.03261176
cg00226923 0.9720684
cg00431549 0.03206336
cg00491404 0.2163383
cg00546897 0.9705579
cg00554250 0.1531578
cg00620024 0.8865442
cg00673191 0.8103681
cg00739120 0.35998
cg00795812 0.9225091
cg00899659 0.6072776
cg01031251 0.9700542
cg01124420 0.6800482
cg01182697 0.2459007
cg01278291 0.7695816
cg01344171 0.8021089
cg01441777 0.5862613

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap