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Sample GSM1320664 Query DataSets for GSM1320664
Status Public on Feb 04, 2014
Title genomic DNA from NKT cells Purified WBC subtype (NKT2574)
Sample type genomic
Source name NKT cells Purified WBC subtype NKT2574
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: NKT cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description NKT2574
NKT cells Purified WBC subtype NKT2574
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.2040848
foxp3DMR1in 0.9484318
foxp3DMRpro 0.5607767
cg00071250 0.04449136
cg00226923 0.959242
cg00431549 0.06250031
cg00491404 0.5150769
cg00546897 0.9498641
cg00554250 0.2376881
cg00620024 0.3428855
cg00673191 0.7282373
cg00739120 0.5610338
cg00795812 0.2143696
cg00899659 0.7543603
cg01031251 0.9484606
cg01124420 0.1515318
cg01182697 0.8353491
cg01278291 0.8516409
cg01344171 0.8304161
cg01441777 0.9215946

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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