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Sample GSM1320660 Query DataSets for GSM1320660
Status Public on Feb 04, 2014
Title genomic DNA from Neutrophils Purified WBC subtype (Neut1148)
Sample type genomic
Source name Neutrophils Purified WBC subtype Neut1148
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Neutrophils
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Neut1148
Neutrophils Purified WBC subtype Neut1148
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9867207
foxp3DMR1in 0.9782192
foxp3DMRpro 0.9582339
cg00071250 0.9231689
cg00226923 0.9748271
cg00431549 0.02633194
cg00491404 0.8594123
cg00546897 0.5866311
cg00554250 0.07116804
cg00620024 0.9165711
cg00673191 0.2401359
cg00739120 0.1238743
cg00795812 0.981465
cg00899659 0.05496694
cg01031251 0.8742352
cg01124420 0.7263392
cg01182697 0.04893389
cg01278291 0.1263228
cg01344171 0.6061401
cg01441777 0.4520544

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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