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Sample GSM1320655 Query DataSets for GSM1320655
Status Public on Feb 04, 2014
Title genomic DNA from Monocytes Purified WBC subtype (Mono1972)
Sample type genomic
Source name Monocytes Purified WBC subtype Mono1972
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Monocytes
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Mono1972
Monocytes Purified WBC subtype Mono1972
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9881371
foxp3DMR1in 0.9786192
foxp3DMRpro 0.9662849
cg00071250 0.9186707
cg00226923 0.9733847
cg00431549 0.02546758
cg00491404 0.8881513
cg00546897 0.08093137
cg00554250 0.06666539
cg00620024 0.9107524
cg00673191 0.7613295
cg00739120 0.2034799
cg00795812 0.5535745
cg00899659 0.03405642
cg01031251 0.9554998
cg01124420 0.7434016
cg01182697 0.04900514
cg01278291 0.3737381
cg01344171 0.5688027
cg01441777 0.3858404

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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