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Sample GSM1320650 Query DataSets for GSM1320650
Status Public on Feb 04, 2014
Title genomic DNA from CD8+ T-cells Purified WBC subtype (Tc2615)
Sample type genomic
Source name CD8+ T-cells Purified WBC subtype Tc2615
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD8+ T-cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Tc2615
CD8+ T-cells Purified WBC subtype Tc2615
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.0317173
foxp3DMR1in 0.9596817
foxp3DMRpro 0.7901459
cg00071250 0.04893545
cg00226923 0.9665443
cg00431549 0.1002254
cg00491404 0.743392
cg00546897 0.972932
cg00554250 0.1387827
cg00620024 0.2062629
cg00673191 0.8454664
cg00739120 0.5225583
cg00795812 0.04464734
cg00899659 0.4922668
cg01031251 0.961683
cg01124420 0.3060046
cg01182697 0.6857005
cg01278291 0.8875281
cg01344171 0.8465737
cg01441777 0.9572608

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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