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Sample GSM1320649 Query DataSets for GSM1320649
Status Public on Feb 04, 2014
Title genomic DNA from CD4+ T-cells Purified WBC subtype (Th1702)
Sample type genomic
Source name CD4+ T-cells Purified WBC subtype Th1702
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: CD4+ T-cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Th1702
CD4+ T-cells Purified WBC subtype Th1702
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.05914558
foxp3DMR1in 0.9443454
foxp3DMRpro 0.6969256
cg00071250 0.02912213
cg00226923 0.9686149
cg00431549 0.1083799
cg00491404 0.7677794
cg00546897 0.9717607
cg00554250 0.1991393
cg00620024 0.08883067
cg00673191 0.8451093
cg00739120 0.4669066
cg00795812 0.06290565
cg00899659 0.9127345
cg01031251 0.9632581
cg01124420 0.07063708
cg01182697 0.6120569
cg01278291 0.8555915
cg01344171 0.7813304
cg01441777 0.9297284

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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