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Sample GSM1320647 Query DataSets for GSM1320647
Status Public on Feb 04, 2014
Title genomic DNA from Pan-T cells Purified WBC subtype (Tpan1754)
Sample type genomic
Source name Pan-T cells Purified WBC subtype Tpan1754
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Pan-T cells
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Tpan1754
Pan-T cells Purified WBC subtype Tpan1754
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.0399729
foxp3DMR1in 0.8878467
foxp3DMRpro 0.7883041
cg00071250 0.02613933
cg00226923 0.9607402
cg00431549 0.09896628
cg00491404 0.5390971
cg00546897 0.9631788
cg00554250 0.2398762
cg00620024 0.1168249
cg00673191 0.7680445
cg00739120 0.5797223
cg00795812 0.0544774
cg00899659 0.9009165
cg01031251 0.9486653
cg01124420 0.1038664
cg01182697 0.7867094
cg01278291 0.87467
cg01344171 0.8404089
cg01441777 0.9369095

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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