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Sample GSM1320641 Query DataSets for GSM1320641
Status Public on Feb 04, 2014
Title genomic DNA from Granulocytes Purified WBC subtype (Gran532)
Sample type genomic
Source name Granulocytes Purified WBC subtype Gran532
Organism Homo sapiens
Characteristics sample type: Purified WBC subtype
disease state: normal
sample subtype: Granulocytes
Treatment protocol Leukocyte subtypes were purified via magnetic activated cell sorting (MACS) using kits from Miltenyi Biotec
Growth protocol Not applicable
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted using QIAGEN Dneasy blood and tissue kit according to standard instructions
Label Cy5 and Cy3
Label protocol Standard Illumina Protocol
Hybridization protocol Bisulphite converted DNA was amplified, fragmented and hybridized to Illumina Infinium Human Methylation27 Beadchip using standard Illumina protocol
Scan protocol Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
Description Gran532
Granulocytes Purified WBC subtype Gran532
Illumina VeraCode GGMA
Data processing Illumina GenomeStudio v2010.3
Unmethylated and methylated signal intensities are reported in a supplementary file on the Series record.
Submission date Feb 04, 2014
Last update date Feb 04, 2014
Contact name William P Accomando
Organization name Harvard School of Public Health
Department Epidemiology
Street address Kresge Bldg 911, 677 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platform ID GPL18246
Series (2)
GSE54647 VeraCode GoldenGate Methylation Assay microarray data for purified human leukocytes, reconstruction mixtures and human whole blood samples
GSE54671 Quantitative reconstruction of leukocyte subsets using DNA methylation

Data table header descriptions
VALUE Average Beta (0-1 constrained), not normalized

Data table
cd3zDMR 0.9833683
foxp3DMR1in 0.9778094
foxp3DMRpro 0.9595407
cg00071250 0.8889635
cg00226923 0.9762617
cg00431549 0.02627565
cg00491404 0.9014148
cg00546897 0.514088
cg00554250 0.08054863
cg00620024 0.9004764
cg00673191 0.2058022
cg00739120 0.1739499
cg00795812 0.976547
cg00899659 0.08772019
cg01031251 0.8295172
cg01124420 0.6825404
cg01182697 0.094814
cg01278291 0.2963601
cg01344171 0.6328967
cg01441777 0.3565493

Total number of rows: 96

Table truncated, full table size 1 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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