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Sample GSM1318060 Query DataSets for GSM1318060
Status Public on Sep 30, 2014
Title Hsp90-alpha KO
Sample type SRA
Source name E16.5 whole testes
Organism Mus musculus
Characteristics strain: C57BL/6
age: embryonic day 16.5
tissue: testis
Treatment protocol 20 whole testes for each genotype
Growth protocol natural mating
Extracted molecule total RNA
Extraction protocol standard acid-phenol extraction by using Isogen (Toyobo)
TruSeq Small RNA Library Preparation Kit (Illumina)
Library strategy RNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina MiSeq
Data processing basecalls performed on MiSeq computer
Sequenced reads were trimmed for adaptor sequence and mapped, using seqmap, to known cellular RNAs (rRNA, tRNA, etc), miRNAs (miRBase), transposon consensus sequences (RepBase), and mm9 whole genome.
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include sequences and their read counts.
Submission date Jan 29, 2014
Last update date May 15, 2019
Contact name Kenji Ichiyanagi
Phone +81-52-789-4066
Organization name Nagoya University
Department Graduate School of Bioagricultural Sciences
Lab Laboratory of Genome and Epigenome Dynamics
Street address Furo-cho, Chikusa-ku
City Nagoya
ZIP/Postal code 464-8601
Country Japan
Platform ID GPL16417
Series (1)
GSE54515 Small RNA analysis for fetal testes of Hsp90-alpha knockout mice
BioSample SAMN02599190
SRA SRX451772

Supplementary file Size Download File type/resource
GSM1318060_Hsp90_KO_E165.txt.gz 3.8 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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