|
Status |
Public on Jan 21, 2014 |
Title |
MeDIP-seq data_30143 |
Sample type |
SRA |
|
|
Source name |
monozygotic twin pair_Control
|
Organism |
Homo sapiens |
Characteristics |
twin pair: 19 subject status: Control tissue: whole blood sample
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Whole blood samples were collected from the twins and stored at -80°C in EDTA tubes. DNA was extracted from 2 x 6ml EDTA blood using the Nucleon Genomic DNA Extraction Kit BACC3 and stored at -20°C in TE Buffer. BGI protocol
|
|
|
Library strategy |
MeDIP-Seq |
Library source |
genomic |
Library selection |
5-methylcytidine antibody |
Instrument model |
Illumina Genome Analyzer |
|
|
Description |
30143
|
Data processing |
Basecalls performed using CASAVA version 1.4 MeDIP-seq reads were aligned to the hg19 genome assembly using bwa Genome_build: hg19 bed files were generated from bam files using the bedtools package Supplementary_files_format_and_content: RPKM files were generated from bed files using the MEDIPS R package
|
|
|
Submission date |
Jan 20, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Matthew N Davies |
Organization name |
King's College London
|
Department |
Twin Research
|
Lab |
Twin Research
|
Street address |
St Thomas' Hospital Campus
|
City |
London |
State/province |
Greater London |
ZIP/Postal code |
SE1 7EH |
Country |
United Kingdom |
|
|
Platform ID |
GPL9052 |
Series (1) |
GSE54222 |
Hypermethylation in the ZBTB20 gene is associated with major depressive disorder |
|
Relations |
BioSample |
SAMN02592835 |
SRA |
SRX443161 |