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Sample GSM1309453 Query DataSets for GSM1309453
Status Public on Jul 31, 2014
Title Patient 18 in group SynSa2 [transcriptome]
Sample type RNA
Source name tumor sample
Organism Homo sapiens
Characteristics histosubtype: synovial sarcoma
age at diagnosis [years]: 25
gender: Female
Treatment protocol not applicable
Growth protocol not applicable
Extracted molecule total RNA
Extraction protocol Total RNA of sufficient integrity and purity was obtained from 57 tumor specimens using the miRNeasy Mini Kit (Qiagen) with optional DNase treatment using RNase-free DNase Set (Qiagen). RNA quality was checked using Bio-Rad Experion system (Bio-Rad Laboratories).
Label Cy3
Label protocol Total RNA (25ng) was reverse transcribed into cDNA by incorporating a T7 oligo-dT promoter primer prior to the generation of fluorescent cRNA using an Agilent LowInput Quick Amp Labeling Kit (Agilent Technologies, SantaClara, CA, USA).
Hybridization protocol Gene expression analysis was performed using Human Gene Expression 4x44K v2 microarrays (G4845A) according to the one-color microarray-based expression analysis protocol (Agilent Technologies).
Scan protocol The microarray slides were scanned using the Agilent G2565A DNA Microarray Scanner (Agilent Technologies, SantaClara, CA, USA).
Description Gene expression in primary tumor sample
Data processing Image analysis was done using the Feature Extraction V10.10.1.1 software (Agilent Technologies, SantaClara, CA, USA). Microarray data were analyzed using Gene Spring GX default workflow parameters for Agilent one-color expression experiments (Agilent Technologies, SantaClara, CA, USA).
Submission date Jan 17, 2014
Last update date Jul 31, 2014
Contact name Joanna Przybyl
Organization name Stanford University
Department Department of Pathology
Street address 300 Pasteur Drive
City Stanford
ZIP/Postal code 94305
Country USA
Platform ID GPL13497
Series (2)
GSE54187 Metastatic potential is determined early in synovial sarcoma development and reflected by tumor molecular features [transcriptome]
GSE54188 Metastatic potential is determined early in synovial sarcoma development and reflected by tumor molecular features

Data table header descriptions
VALUE Normalized signal intensity (Quantile normalization)

Data table
GE_BrightCorner 0.2977805
DarkCorner 0.42519474
A_23_P146146 0.31057787
A_23_P42935 0.76563597
A_23_P117082 0.675838
A_23_P2683 -0.09192324
A_24_P358131 -1.1191568
A_33_P3367647 0.2656355
A_23_P157316 -1.8001347
A_32_P14850 -0.05646372
A_23_P158596 0.0
A_23_P350107 0.8428979
A_23_P388190 -0.23657656
A_23_P106544 -0.1103282
A_33_P3219745 -0.375432
A_32_P85539 -0.03342676
A_23_P94998 0.0
A_33_P3235677 -0.31539822
A_23_P417014 0.0
A_23_P103905 -0.14136791

Total number of rows: 34183

Table truncated, full table size 797 Kbytes.

Supplementary file Size Download File type/resource
GSM1309453_SynSa2_18.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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