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Sample GSM1309446 Query DataSets for GSM1309446
Status Public on Jul 31, 2014
Title Patient 9 in group SynSa2 [transcriptome]
Sample type RNA
 
Source name tumor sample
Organism Homo sapiens
Characteristics histosubtype: synovial sarcoma
age at diagnosis [years]: 24
gender: Male
Treatment protocol not applicable
Growth protocol not applicable
Extracted molecule total RNA
Extraction protocol Total RNA of sufficient integrity and purity was obtained from 57 tumor specimens using the miRNeasy Mini Kit (Qiagen) with optional DNase treatment using RNase-free DNase Set (Qiagen). RNA quality was checked using Bio-Rad Experion system (Bio-Rad Laboratories).
Label Cy3
Label protocol Total RNA (25ng) was reverse transcribed into cDNA by incorporating a T7 oligo-dT promoter primer prior to the generation of fluorescent cRNA using an Agilent LowInput Quick Amp Labeling Kit (Agilent Technologies, SantaClara, CA, USA).
 
Hybridization protocol Gene expression analysis was performed using Human Gene Expression 4x44K v2 microarrays (G4845A) according to the one-color microarray-based expression analysis protocol (Agilent Technologies).
Scan protocol The microarray slides were scanned using the Agilent G2565A DNA Microarray Scanner (Agilent Technologies, SantaClara, CA, USA).
Description Gene expression in primary tumor sample
Data processing Image analysis was done using the Feature Extraction V10.10.1.1 software (Agilent Technologies, SantaClara, CA, USA). Microarray data were analyzed using Gene Spring GX default workflow parameters for Agilent one-color expression experiments (Agilent Technologies, SantaClara, CA, USA).
 
Submission date Jan 17, 2014
Last update date Jul 31, 2014
Contact name Joanna Przybyl
E-mail(s) jprzybyl@stanford.edu
Organization name Stanford University
Department Department of Pathology
Street address 300 Pasteur Drive
City Stanford
ZIP/Postal code 94305
Country USA
 
Platform ID GPL13497
Series (2)
GSE54187 Metastatic potential is determined early in synovial sarcoma development and reflected by tumor molecular features [transcriptome]
GSE54188 Metastatic potential is determined early in synovial sarcoma development and reflected by tumor molecular features

Data table header descriptions
ID_REF
VALUE Normalized signal intensity (Quantile normalization)

Data table
ID_REF VALUE
GE_BrightCorner -0.6604557
DarkCorner -0.5640173
A_23_P146146 1.7151141
A_23_P42935 0.0
A_23_P117082 0.0
A_23_P2683 0.026372433
A_24_P358131 2.7315316
A_33_P3367647 -0.036053658
A_23_P157316 1.0227089
A_32_P14850 0.81608486
A_23_P158596 0.553185
A_23_P350107 0.24214745
A_23_P388190 -0.00663805
A_23_P106544 0.41057682
A_33_P3219745 1.3607926
A_32_P85539 -0.87488174
A_23_P94998 0.6162286
A_33_P3235677 1.4891791
A_23_P417014 1.1234975
A_23_P103905 0.49299955

Total number of rows: 34183

Table truncated, full table size 793 Kbytes.




Supplementary file Size Download File type/resource
GSM1309446_SynSa2_9.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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