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Sample GSM1308543 Query DataSets for GSM1308543
Status Public on Mar 21, 2016
Title Control rep3
Sample type SRA
Source name control_myocardium
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
mouse weight: 250-280g
gender: male
sample group: control
tissue: heart; myocardium
Extracted molecule total RNA
Extraction protocol Hearts were removed, flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description Sample 8
Data processing Illumina Casava1.8.2 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to UCSC RN4 whole genome using tophat v2.0.6
Differential gene and transcript expression analysis using a protocol from Cole Trapnell et al., nature protocols, 2012.
Genome_build: Rattus_norvegicus_UCSC_rn4
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample .
Submission date Jan 16, 2014
Last update date May 15, 2019
Contact name Bing-Mei Zhu
Phone +86-025-85811235
Organization name Nanjing university of Chinese Medicine
Street address 138 Xianlin Avenue
City Nanjing
State/province Jiangsu
ZIP/Postal code 210023
Country China
Platform ID GPL14844
Series (1)
GSE54132 Genome-wide profiling of gene expression under electro-acupuncture treatment on MI heart in rats
BioSample SAMN02585242
SRA SRX434339

Supplementary file Size Download File type/resource
GSM1308543_ctr3_genes.fpkm_tracking.gz 374.8 Kb (ftp)(http) FPKM_TRACKING
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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