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Sample GSM1304966 Query DataSets for GSM1304966
Status Public on Jan 11, 2014
Title MDD_pfc_3
Sample type RNA
 
Source name Pre-frontal cortex (BA46), major depressive disorder
Organism Homo sapiens
Characteristics age: 40
gender: F
race: W
pmi: 22.2
ph: 6.6
rin: 7.7
tissue: Pre-frontal cortex (BA46)
disease state: major depressive disorder
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Five (5) ug of fragmented, biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133_Plus 2 Gene Chips for 18 hours, then washed/stained according to manufacturer's recommendations using the Affymetrix 450 fluidics station.
Scan protocol Gene chips were scanned using an Affymetrix 3000 array scanner.
Data processing R/Bioconductor was used to apply the Robust Multi-Array Average (RMA) methodology to generate expression values. Brain regions were normalized separately.
 
Submission date Jan 10, 2014
Last update date Jan 11, 2014
Contact name Thomas A Lanz
E-mail(s) thomas.a.lanz@pfizer.com
Phone 860-441-3551
Organization name Pfizer
Department Global Pathology and Investigative Toxicology
Street address Eastern Point Rd MS# 8274-1224
City Groton
State/province CT
ZIP/Postal code 06340
Country USA
 
Platform ID GPL570
Series (1)
GSE53987 Microarray profiling of PFC, HPC and STR from subjects with schizophrenia, bipolar, MDD or control

Data table header descriptions
ID_REF
VALUE RMA expression values

Data table
ID_REF VALUE
1007_s_at 849.24
1053_at 101.43
117_at 22.89
121_at 227.24
1255_g_at 39.7
1294_at 47.93
1316_at 163.2
1320_at 15.41
1405_i_at 10.07
1431_at 31.85
1438_at 58.46
1487_at 142.53
1494_f_at 38.46
1552256_a_at 166.96
1552257_a_at 255.71
1552258_at 16.09
1552261_at 14.75
1552263_at 11.33
1552264_a_at 112.56
1552266_at 9.36

Total number of rows: 54675

Table truncated, full table size 901 Kbytes.




Supplementary file Size Download File type/resource
GSM1304966_3_MDD_B46.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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