|
| Status |
Public on Jan 11, 2014 |
| Title |
bipolar_pfc_4 |
| Sample type |
RNA |
| |
|
| Source name |
Pre-frontal cortex (BA46), bipolar disorder
|
| Organism |
Homo sapiens |
| Characteristics |
age: 45
gender: M
race: W
pmi: 27.2
ph: 7.1
rin: 7.3
tissue: Pre-frontal cortex (BA46)
disease state: bipolar disorder
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Five (5) ug of fragmented, biotin-labeled cDNA was hybridized to Affymetrix Human Genome U133_Plus 2 Gene Chips for 18 hours, then washed/stained according to manufacturer's recommendations using the Affymetrix 450 fluidics station.
|
| Scan protocol |
Gene chips were scanned using an Affymetrix 3000 array scanner.
|
| Data processing |
R/Bioconductor was used to apply the Robust Multi-Array Average (RMA) methodology to generate expression values. Brain regions were normalized separately.
|
| |
|
| Submission date |
Jan 10, 2014 |
| Last update date |
Jan 11, 2014 |
| Contact name |
Thomas A Lanz |
| E-mail(s) |
thomas.a.lanz@pfizer.com
|
| Phone |
860-441-3551
|
| Organization name |
Pfizer
|
| Department |
Global Pathology and Investigative Toxicology
|
| Street address |
Eastern Point Rd MS# 8274-1224
|
| City |
Groton |
| State/province |
CT |
| ZIP/Postal code |
06340 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE53987 |
Microarray profiling of PFC, HPC and STR from subjects with schizophrenia, bipolar, MDD or control |
|
