|
Status |
Public on Mar 21, 2014 |
Title |
Slp-1 |
Sample type |
RNA |
|
|
Source name |
Bone marrow B-cell fraction, B220+CD19+ pre-B
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 cell type: pre-B cell genotype/variation: Slp KO (VH81x transgenic Rag1-/-)
|
Treatment protocol |
RNA was extracted after one PBS wash
|
Growth protocol |
developing B cells were isolated directly from the bone marrow by FACS purification and processed for analysis.
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA
|
Label |
biotin
|
Label protocol |
Total RNA was labeled using the Affymetrix GeneChip® WT (Whole Transcript) Terminal Labeling Kit, according to manufacturers instructions
|
|
|
Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the mouse gene 1.0st array in a GeneChip Hyb oven 640 according to manufacturer instructions. Washing was done in a GeneChip WS-system according to manufacturer instructions.
|
Scan protocol |
Scanning was done on a geneChip scanner 7G incl. autocaroussel using affymetrix launcher software for downstream image processing
|
Description |
pre-B cells, from VH81x transgenic Rag1-/- mice
|
Data processing |
Normalized gene expression values were calculated using robust multi-array average (RMA)
|
|
|
Submission date |
Jan 08, 2014 |
Last update date |
Mar 21, 2014 |
Contact name |
Ralph Stadhouders |
E-mail(s) |
stadhoudersralph@gmail.com
|
Organization name |
Erasmus Medical Center
|
Department |
Pulmonary Medicine
|
Street address |
Dr. Molewaterplein 50
|
City |
Rotterdam |
ZIP/Postal code |
3015 GE |
Country |
Netherlands |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE53896 |
Pre-BCR Signaling induce IgK Locus Accessibility by functional redistribution of Enhancer-mediated chromatin Interactions |
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