|
| Status |
Public on Dec 31, 2013 |
| Title |
Tau, Input round #1 |
| Sample type |
SRA |
| |
|
| Source name |
Tau, Input
|
| Organism |
Drosophila melanogaster |
| Characteristics |
age: 10 days old
genotype/variation: elav-GAL4/Y;UAS-tauR406W
Sex: male
tissue: whole heads
chip antibody: none
chip antibody vendor: Abcam
chip antibody cat. #: ab1220
molecule subtype: input genomice DNA
|
| Growth protocol |
Drosophila melanogaster crosses and aging were performed at 25°C. The pan-neuronal elav-GAL4 driver was used to drive tauR406W transgene expression.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
2 grams of fly heads were ground in liquid nitrogen and DNA was crosslinked to protein with formaldehyde. Chromatin was sheared to 500 bp prior to ChIP
Libraries were prepared using the NEBNext ChIP-seq Library Prep Reagent Set for Illumina according to the manufacturer's recommendations
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
ChIP-seq reads were aligned to the Drosophila reference genome using Bowtie
PCR duplicates were removed
Reads were extended to 350 base pairs
Total read count was normalized to 10,000,000 reads
Input sequences were subtracted from ChIPped sequences
Genome_build: Dmel Release 5
Supplementary_files_format_and_content: Processed bw files are suitable for upload into genome browsers
|
| |
|
| Submission date |
Dec 30, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Bess Frost |
| E-mail(s) |
sefrost@partners.org
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Pathology
|
| Lab |
Mel Feany
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE53719 |
Tau promotes neurodegeneration through global chromatin relaxation |
|
| Relations |
| BioSample |
SAMN02487083 |
| SRA |
SRX403439 |
