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Sample GSM1299406 Query DataSets for GSM1299406
Status Public on Dec 31, 2013
Title Tau, ChIP-seq round #1
Sample type SRA
 
Source name Tau, ChIP-seq
Organism Drosophila melanogaster
Characteristics age: 10 days old
genotype/variation: elav-GAL4/Y;UAS-tauR406W
Sex: male
tissue: whole heads
chip antibody: H3K9me2
chip antibody vendor: Abcam
chip antibody cat. #: ab1220
molecule subtype: H3K9me2 ChIPed DNA
Growth protocol Drosophila melanogaster crosses and aging were performed at 25°C. The pan-neuronal elav-GAL4 driver was used to drive tauR406W transgene expression.
Extracted molecule genomic DNA
Extraction protocol 2 grams of fly heads were ground in liquid nitrogen and DNA was crosslinked to protein with formaldehyde. Chromatin was sheared to 500 bp prior to ChIP
Libraries were prepared using the NEBNext ChIP-seq Library Prep Reagent Set for Illumina according to the manufacturer's recommendations
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Data processing ChIP-seq reads were aligned to the Drosophila reference genome using Bowtie
PCR duplicates were removed
Reads were extended to 350 base pairs
Total read count was normalized to 10,000,000 reads
Input sequences were subtracted from ChIPped sequences
Genome_build: Dmel Release 5
Supplementary_files_format_and_content: Processed bw files are suitable for upload into genome browsers
 
Submission date Dec 30, 2013
Last update date May 15, 2019
Contact name Bess Frost
E-mail(s) sefrost@partners.org
Organization name Brigham and Women's Hospital
Department Pathology
Lab Mel Feany
Street address 77 Avenue Louis Pasteur
City Boston
State/province Massachusetts
ZIP/Postal code 02115
Country USA
 
Platform ID GPL13304
Series (1)
GSE53719 Tau promotes neurodegeneration through global chromatin relaxation
Relations
BioSample SAMN02487076
SRA SRX403435

Supplementary file Size Download File type/resource
GSM1299406_1_Tau_H3K9me2_enriched.bw 50.3 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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