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Sample GSM1298089 Query DataSets for GSM1298089
Status Public on Dec 27, 2013
Title Luc-Tanoue
Sample type RNA
Source name Peripheral Blood
Organism Homo sapiens
Characteristics phenotyp: B-ALL line
cell line: Tanoue
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNeasy Mini Kit (QIAGEN), according to the protocol provided by the manufacturer.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the Low Input Quick Amplification Labeling kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA).
Hybridization protocol 0.6 ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a reaction volume of 25 μl containing 25x Agilent fragmentation buffer following the manufacturers instructions. On completion of the fragmentation reaction, 25 μl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 8x60k array slides
Description Gene expression after 6hr in 0.5Gy-irradiated human blood
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent)
Submission date Dec 26, 2013
Last update date Dec 27, 2013
Contact name takashi kondo
Organization name Sapporo Medical University
Department Clinical Laboratory Medicine
Street address South-1, West-16, Chuo-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8543
Country Japan
Platform ID GPL13607
Series (1)
GSE53651 CD7 promotes extramedullary involvement of the B-cell acute lymphoblastic leukemia line Tanoue by enhancing integrin beta2-dependent cell adhesiveness

Data table header descriptions
VALUE Agilent default normalized signal intensity

Data table
1 175.6647975
2 0.012173949
3 0.01221092
4 0.641367164
5 0.84625303
6 0.295330979
7 7.348738932
8 4.475569277
9 0.01233748
10 0.139943114
11 0.012544641
12 3.338761639
13 0.022019303
14 1.65168761
15 35.81927918
16 0.012341294
17 0.439792132
18 0.012319442
19 0.012303017
20 2.417285539

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM1298089_US82800151_252800417050_S01_GE1_107_Sep09_1_2.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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