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Sample GSM1296901 Query DataSets for GSM1296901
Status Public on Dec 24, 2013
Title HaCaT_40hr_muTRPV3
Sample type RNA
 
Source name HaCaT, transfected with mutant TRPV3, 40hr
Organism Homo sapiens
Characteristics cell line: HaCaT
transfection: mutant TRPV3
time: 40 hrs
Treatment protocol For TRPV3 mutant overexpression, HaCaT cells were transfected in 6-well dishes at approximately 70% confluence using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. Control cells were treated with the same plasmid without insert.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
Label Cy3
Label protocol Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
 
Hybridization protocol The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
Scan protocol The arrays were washed and scanned using an Agilent scanner.
Description Gene expression of HaCaT cell lines at 40hr, transfected with mutant TRPV3
Data processing The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
 
Submission date Dec 23, 2013
Last update date Dec 24, 2013
Contact name Ji-Hoon Cho
E-mail(s) jcho@systemsbiology.org
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL17077
Series (2)
GSE53615 Gene expression signatures of HaCaT cell lines transfected with mutant TRPV3
GSE53616 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3

Data table header descriptions
ID_REF
VALUE Quantile normalized and log2-transformed gene expression values.

Data table
ID_REF VALUE
GE_BrightCorner 15.91408009
DarkCorner 5.419932604
A_23_P117082 12.12050727
A_33_P3246448 6.035257325
A_33_P3318220 5.486499862
A_33_P3236322 5.768184325
A_33_P3319925 6.515699838
A_21_P0000509 16.58063398
A_21_P0000744 6.860052345
A_24_P215804 9.422765614
A_23_P110167 11.52955951
A_33_P3211513 7.786814144
A_23_P103349 5.756691728
A_32_P61480 5.619608644
A_33_P3788124 5.297680549
A_33_P3414202 7.853829352
A_33_P3316686 7.835524112
A_33_P3300975 9.358010105
A_33_P3263061 10.67154107
A_33_P3261373 5.318919345

Total number of rows: 50739

Table truncated, full table size 1259 Kbytes.




Supplementary file Size Download File type/resource
GSM1296901_HaCaT_40hr_muTRPV3.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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