|Public on Dec 24, 2013
|HaCaT, transfected with mutant TRPV3, 16hr
|cell line: HaCaT
transfection: mutant TRPV3
time: 16 hrs
|For TRPV3 mutant overexpression, HaCaT cells were transfected in 6-well dishes at approximately 70% confluence using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. Control cells were treated with the same plasmid without insert.
|Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
|Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
|The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
|The arrays were washed and scanned using an Agilent scanner.
|Gene expression of HaCaT cell lines at 16hr, transfected with mutant TRPV3
|The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
|Dec 23, 2013
|Last update date
|Dec 24, 2013
|Institute for Systems Biology
|401 Terry Avenue North
|Gene expression signatures of HaCaT cell lines transfected with mutant TRPV3
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