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Sample GSM1296899 Query DataSets for GSM1296899
Status Public on Dec 24, 2013
Title HaCaT_16hr_muTRPV3
Sample type RNA
Source name HaCaT, transfected with mutant TRPV3, 16hr
Organism Homo sapiens
Characteristics cell line: HaCaT
transfection: mutant TRPV3
time: 16 hrs
Treatment protocol For TRPV3 mutant overexpression, HaCaT cells were transfected in 6-well dishes at approximately 70% confluence using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. Control cells were treated with the same plasmid without insert.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
Label Cy3
Label protocol Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
Hybridization protocol The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
Scan protocol The arrays were washed and scanned using an Agilent scanner.
Description Gene expression of HaCaT cell lines at 16hr, transfected with mutant TRPV3
Data processing The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
Submission date Dec 23, 2013
Last update date Dec 24, 2013
Contact name Ji-Hoon Cho
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platform ID GPL17077
Series (2)
GSE53615 Gene expression signatures of HaCaT cell lines transfected with mutant TRPV3
GSE53616 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3

Data table header descriptions
VALUE Quantile normalized and log2-transformed gene expression values.

Data table
GE_BrightCorner 15.80369267
DarkCorner 5.439532513
A_23_P117082 11.80525841
A_33_P3246448 6.05166212
A_33_P3318220 5.297680549
A_33_P3236322 5.74819285
A_33_P3319925 6.392317423
A_21_P0000509 16.66376308
A_21_P0000744 6.632995197
A_24_P215804 9.437231901
A_23_P110167 11.36245123
A_33_P3211513 7.591210648
A_23_P103349 5.812616062
A_32_P61480 5.626925794
A_33_P3788124 5.232021123
A_33_P3414202 7.646858678
A_33_P3316686 8.087462841
A_33_P3300975 8.680359524
A_33_P3263061 10.40788735
A_33_P3261373 5.564784619

Total number of rows: 50739

Table truncated, full table size 1259 Kbytes.

Supplementary file Size Download File type/resource
GSM1296899_HaCaT_16hr_muTRPV3.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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