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Sample GSM1296898 Query DataSets for GSM1296898
Status Public on Dec 24, 2013
Title HaCaT_40hr_Control
Sample type RNA
Source name HaCaT, Control, 40hr
Organism Homo sapiens
Characteristics cell line: HaCaT
transfection: control
time: 40 hrs
Treatment protocol For TRPV3 mutant overexpression, HaCaT cells were transfected in 6-well dishes at approximately 70% confluence using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. Control cells were treated with the same plasmid without insert.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
Label Cy3
Label protocol Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
Hybridization protocol The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
Scan protocol The arrays were washed and scanned using an Agilent scanner.
Description Gene expression of HaCaT cell lines at 40hr, Control
Data processing The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
Submission date Dec 23, 2013
Last update date Dec 24, 2013
Contact name Ji-Hoon Cho
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platform ID GPL17077
Series (2)
GSE53615 Gene expression signatures of HaCaT cell lines transfected with mutant TRPV3
GSE53616 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3

Data table header descriptions
VALUE Quantile normalized and log2-transformed gene expression values.

Data table
GE_BrightCorner 15.60125346
DarkCorner 5.587638663
A_23_P117082 12.58998096
A_33_P3246448 6.255815423
A_33_P3318220 5.475733431
A_33_P3236322 6.040746342
A_33_P3319925 6.589963182
A_21_P0000509 16.06620834
A_21_P0000744 7.30833903
A_24_P215804 8.995649394
A_23_P110167 11.86186318
A_33_P3211513 7.515042724
A_23_P103349 5.888143471
A_32_P61480 5.914883386
A_33_P3788124 5.502500341
A_33_P3414202 8.070568222
A_33_P3316686 8.121965139
A_33_P3300975 9.780676458
A_33_P3263061 11.29342375
A_33_P3261373 5.761551232

Total number of rows: 50739

Table truncated, full table size 1259 Kbytes.

Supplementary file Size Download File type/resource
GSM1296898_HaCaT_40hr_Control.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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