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Sample GSM1296894 Query DataSets for GSM1296894
Status Public on Dec 24, 2013
Title 293T_24hr_muTRPV3
Sample type RNA
 
Source name 293T, transfected with mutant TRPV3, 24hr
Organism Homo sapiens
Characteristics cell line: 293T
transfection: mutant TRPV3
time: 24 hrs
Treatment protocol For TRPV3 mutant overexpression, 293T cells were transfected in 6-well dishes at approximately 70% confluence using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. Control cells were treated with the same plasmid without insert.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
Label Cy3
Label protocol Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
 
Hybridization protocol The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
Scan protocol The arrays were washed and scanned using an Agilent scanner.
Description Gene expression of 293T cell lines at 24hr, transfected with mutant TRPV3
Data processing The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
 
Submission date Dec 23, 2013
Last update date Dec 24, 2013
Contact name Ji-Hoon Cho
E-mail(s) jcho@systemsbiology.org
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL17077
Series (2)
GSE53614 Gene expression signatures of 293T cell lines transfected with mutant TRPV3
GSE53616 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3

Data table header descriptions
ID_REF
VALUE Quantile normalized and log2-transformed gene expression values.

Data table
ID_REF VALUE
GE_BrightCorner 16.15630552
DarkCorner 5.512478138
A_23_P117082 12.34819251
A_33_P3246448 6.312882955
A_33_P3318220 5.653442239
A_33_P3236322 6.080373416
A_33_P3319925 6.855906667
A_21_P0000509 18.0798311
A_21_P0000744 11.42377249
A_24_P215804 10.1620241
A_23_P110167 11.93571151
A_33_P3211513 9.192909219
A_23_P103349 6.115613158
A_32_P61480 5.90488546
A_33_P3788124 5.594946589
A_33_P3414202 8.671393635
A_33_P3316686 9.087020771
A_33_P3300975 11.78248638
A_33_P3263061 11.98367651
A_33_P3261373 5.761551232

Total number of rows: 50739

Table truncated, full table size 1258 Kbytes.




Supplementary file Size Download File type/resource
GSM1296894_293T_24hr_muTRPV3.txt.gz 12.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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