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Sample GSM1296893 Query DataSets for GSM1296893
Status Public on Dec 24, 2013
Title 293T_16hr_muTRPV3
Sample type RNA
Source name 293T, transfected with mutant TRPV3, 16hr
Organism Homo sapiens
Characteristics cell line: 293T
transfection: mutant TRPV3
time: 16 hrs
Treatment protocol For TRPV3 mutant overexpression, 293T cells were transfected in 6-well dishes at approximately 70% confluence using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. Control cells were treated with the same plasmid without insert.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
Label Cy3
Label protocol Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
Hybridization protocol The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
Scan protocol The arrays were washed and scanned using an Agilent scanner.
Description Gene expression of 293T cell lines at 16hr, transfected with mutant TRPV3
Data processing The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
Submission date Dec 23, 2013
Last update date Dec 24, 2013
Contact name Ji-Hoon Cho
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platform ID GPL17077
Series (2)
GSE53614 Gene expression signatures of 293T cell lines transfected with mutant TRPV3
GSE53616 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3

Data table header descriptions
VALUE Quantile normalized and log2-transformed gene expression values.

Data table
GE_BrightCorner 16.13358807
DarkCorner 5.546093243
A_23_P117082 12.28544818
A_33_P3246448 6.909893084
A_33_P3318220 5.95419631
A_33_P3236322 6.588714636
A_33_P3319925 7.154818109
A_21_P0000509 18.04961106
A_21_P0000744 11.38140776
A_24_P215804 9.720101721
A_23_P110167 11.76059969
A_33_P3211513 8.786814144
A_23_P103349 6.328389641
A_32_P61480 6.402301511
A_33_P3788124 5.996238081
A_33_P3414202 8.758500843
A_33_P3316686 8.947758679
A_33_P3300975 12.14481754
A_33_P3263061 11.85895231
A_33_P3261373 6.273018494

Total number of rows: 50739

Table truncated, full table size 1260 Kbytes.

Supplementary file Size Download File type/resource
GSM1296893_293T_16hr_muTRPV3.txt.gz 12.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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