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Sample GSM1296889 Query DataSets for GSM1296889
Status Public on Dec 24, 2013
Title III-7_Unaffected_Skin
Sample type RNA
Source name Skin biopsy from III-7, Unaffected
Organism Homo sapiens
Characteristics tissue: skin
source: unaffected skin from III-7
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
Label Cy3
Label protocol Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
Hybridization protocol The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
Scan protocol The arrays were washed and scanned using an Agilent scanner.
Description unaffected skin from III-7
Data processing The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
Submission date Dec 23, 2013
Last update date Dec 24, 2013
Contact name Ji-Hoon Cho
Organization name Institute for Systems Biology
Street address 401 Terry Avenue North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
Platform ID GPL17077
Series (2)
GSE53613 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients
GSE53616 Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3

Data table header descriptions
VALUE Quantile normalized and log2-transformed gene expression values.

Data table
GE_BrightCorner 14.61988847
DarkCorner 5.630373292
A_23_P117082 11.15670257
A_33_P3246448 7.233619677
A_33_P3318220 5.495855027
A_33_P3236322 5.839203788
A_33_P3319925 6.082149041
A_21_P0000509 13.60696902
A_21_P0000744 7.826151215
A_24_P215804 5.915879379
A_23_P110167 12.49453069
A_33_P3211513 8.632086413
A_23_P103349 5.750029222
A_32_P61480 5.527477006
A_33_P3788124 5.686500527
A_33_P3414202 8.187970592
A_33_P3316686 8.145295335
A_33_P3300975 7.178664851
A_33_P3263061 11.97808477
A_33_P3261373 5.791162889

Total number of rows: 50739

Table truncated, full table size 1259 Kbytes.

Supplementary file Size Download File type/resource
GSM1296889_III-7_Unaffected_Skin.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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